日本輸血学会雑誌
Online ISSN : 1883-8383
Print ISSN : 0546-1448
ISSN-L : 0546-1448
自動凍結保存装置 BioArchive を用いた臍帯血凍結保存
笹山 典久和田 誠一須郷 美智子斉藤 健一徐 岩文浅野 茂隆高橋 恒夫
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ジャーナル フリー

2001 年 47 巻 1 号 p. 15-21

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Quality control for the cryopreservation of thousands of units of placental/umbilical cord blood (PCB) is a difficult problem in cord blood banking. The Bio Archive System (BA) is a liquid nitrogen freezer tank equipped with a computer for controlled-rate freezing and a robotic arm for insertion and retrieval of the units. It is designed for the cryopreservation of up to 3, 626 units of PCB. Leukocyte concentrates prepared by the hydroxyethyl starch method were expressed into freezing bags, with large and small compartments, and then frozen and stored in the BA. The recoveries of CD34 positive cells in the large and small compartments were 95.1±9.6% and 93.5±1.6%, respectively (n=8) and the recoveries of colony-forming cell were 99.6±18.5% and 115.8±27.8%, respectively. The results indicate that the BA is useful for controlled cryopreservation of PCB in cord blood banking.

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