An observation was made on the influence of osmotic pressure and pH to the leucocytes outside of the body.
The solutions used were saline solution from 2.0% to 0% and isotonic saline solution with pH 5,6,7and 8.
With low osmotic pressure, distinct destruction of leucocyte, and with high osmotic pressure, promotion of degeneration were recognized.
In 0.6% to 1.2% saline solution, motility of neutrophiles was maintained, and the most long-lasting motility was recognized in 0.9% saline solution. Concerning to pH, in order of pH 5,8,6 and 7, the increase of motility was recognized.