緑膿菌の色素産生に関する研究

殊にピオシアニン株No .134の産生する螢光物質について

抄録

The pigment and fluorescent substances produced by Pseudomonas aeruginosa, strain No.134, in glutamic acid liquid medium were fractionated by gel filtration, and the presence of pyocyanin and of the snbstances corresponding to the Fluorescence I and II were demonstrated.
The apsorbtion spectrum of fluorescence I of strain No.134 shows a peak at 460mμ. A reversible change in the fluorescent color of Fluorescence I fraction takes place between pH 5.0 and pH 10.0. A remarkable change is noted in the absorption spectrum as well as characteristic wave length at about pHs 5.0∼4.0 when the tone of the fluorescence changes unreversibly from colorless to orange.
The paper electrophoretic analysis was perform ed on the Fluorescence I fraction of strains No.134 grown in the glutamic acid lipuid medium and three fluorescent spots were demonstrated. These spots appear in oder from the negative pole side with time of cultivation.
The fluorescence II fraction shows seven fluorescent spots on the filter paper by electrophoresis. Three of them were purely isolated by starch zone electrophoresis and examined spectrophotometrically.