Abstract
Biological actions of hormones are so complicated in vivo that the initial effect of them to the living cells can be recognized exactly, because hormones are influenced by each other in the living being. Direct actions of hormones upon the cultured cells in vitro must be investigated in order to detect the initial effect of them. But, it is difficult to apply steroid hormone into the mediums for tissue cultures because of its solubility. In this point of view, this investigation was carried out and the references of the subject were reviewed.
The emulsions of such steroid hormones as estradiol, stilbestrol, progesterone, testosterone propionate, cortisone and desoxycorticosteronre were prepared by means of Tween 80. The inhibitory effects of them on the fibroblasts from the heart of chick embryo were detected. For the controls Tween 80 and emulsion of sesame oil were observed in the several concentrations.
The water-soluble estrogenic substance, sodium-p-(3-p-hydroxyphenyl)-4-n-hexyl)phenyloxyacetate, was applied into the mediums for tissue cultures. This chemical compound has strong estrogenic activity(5γ=1 r. u. )It was distinguished by the growth index and the phase contrast microscopic observations that the cultured liver cells and the fibroblasts from the heart of chick embryo were inhibited by the cytotoxic effect of this compound.
