We performed physical therapy after fixation of the anterior cruciate ligament (ACL) using fibrin adhesive (Tisseel). It was considered that the tension strength of the reconstructed ligament using Tisseel was weak in the restoration process. Therefore, we thought that prevention of repeat injury to the reconstructed ligament was important. However, the protocol for physical therapy after ligament reconstruction using Tisseel has not been established. Therefore, the mechanical stress on the ligament was forecast from a dynamic alignment in the squatting position. In physical therapy, we gave particular priority to muscle strengthening exercises to stabilize the unstable joint. As a result, the functional disorder safely improved without further injury to the unstable joint, and the subject successfully returned to sports activities.