Journal of Medical and Dental Sciences
Online ISSN : 2185-9132
Print ISSN : 1342-8810
ISSN-L : 1342-8810
Optimal initial cell density that yields the highest number of primary synovial mesenchymal stem cells in a clinical setting
Kiyotaka HoriuchiMitsuru MizunoHisako KatanoKentaro EndoNobutake OzekiKunikazu TsujiHideyuki KogaIchiro Sekiya
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ジャーナル オープンアクセス

2021 年 68 巻 p. 17-26

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Synovial mesenchymal stem cell (MSC) transplantation improved clinical outcomes in knees. In clinical situations, nucleated cells were cultured with 10% autologous serum for 14 days. Nucleated cell number and serum volume practically varied by patient, and initial cell density and dish number affected yield/donor. Our purpose was to estimate the MSC yield/donor from the fold increase obtained in this experiment in several patterns. We obtained the data of the autologous serum volume, nucleated cell number, and initial plating cell density from 10 patients who participated in our previous clinical studies. We used these previous clinical data and the current experimental data. Experimentally, fold increase significantly decreased between 700 and 1,900 cells/cm². In cases where the nucleated cell number was low and the serum volume was high, the estimated MSC yield/donor decreased along with the initial plating density. In cases where the nucleated cell number was high and the serum volume was low, the estimated MSC yield/donor increased along with the initial plating density. In cases where some nucleated cells were discarded, the maximum MSC yield/donor were obtained when the initial cell density was the highest cell density where some nucleated cells were discarded.

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