No relationship exists between PBP 2a amounts expressed in different MRSA strains obtained clinically and their β-lactam MIC values

Md. Anowar Khasru Parvez; Hirofumi Shibata; Tatsuro Nakano; Shingo Niimi; Nobuo Fujii; Naokatu Arakaki; Tomihiko Higuti

doi:10.2152/jmi.55.246

Originals

No relationship exists between PBP 2a amounts expressed in different MRSA strains obtained clinically and their β-lactam MIC values

Md. Anowar Khasru Parvez, Hirofumi Shibata, Tatsuro Nakano, Shingo Niimi, Nobuo Fujii, Naokatu Arakaki, Tomihiko Higuti

Author information

Md. Anowar Khasru Parvez
Department of Molecular Cell Biology and Medicine, Institute of Health Biosciences, the University of Tokushima Graduate School
Hirofumi Shibata
Department of Molecular Cell Biology and Medicine, Institute of Health Biosciences, the University of Tokushima Graduate School
Tatsuro Nakano
Department of Molecular Cell Biology and Medicine, Institute of Health Biosciences, the University of Tokushima Graduate School
Shingo Niimi
Division of Biological Chemistry and Biologicals, National Institute of Health Sciences
Nobuo Fujii
Department of Media Design, Faculty of Human Life Sciences, Tokushima Bunri University
Naokatu Arakaki
Department of Molecular Cell Biology and Medicine, Institute of Health Biosciences, the University of Tokushima Graduate School
Tomihiko Higuti
Department of Molecular Cell Biology and Medicine, Institute of Health Biosciences, the University of Tokushima Graduate School

Keywords: Methicillin-resistant Staphylococcus aureus (MRSA), Penicillin-binding protein (PBP) 2a, β-lactam MIC values

JOURNAL FREE ACCESS

2008 Volume 55 Issue 3,4 Pages 246-253

DOI https://doi.org/10.2152/jmi.55.246

Details

Abstract

After establishing a linear relationship between the amount of penicillin-binding protein (PBP) 2a and membrane proteins of methicillin-resistant Staphylococcus aureus (MRSA) COL by dot-blot analysis using an antibody against PBP 2a, we determined the PBP 2a quantities in membrane fractions prepared from 14 different MRSA cells. Methicillin-sensitive S. aureus ATCC 6538P was used as a quality control strain. The amounts of PBP 2a diverged among the strains, and no relationship to β-lactam MIC values were observed in the corresponding strains. J. Med. Invest. 55: 246-253, August, 2008

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