順天堂醫事雑誌
Online ISSN : 2188-2126
Print ISSN : 2187-9737
ISSN-L : 2187-9737
Poster Sessions - Muscle Metabolism and Endocrine System
Effects of a High-fat Diet on Mitochondrial Fission-Related Protein Drp1 in Slow- and Fast-Twitch Muscles
DAIKI NAKANONORIAKI KAWANISHIKANA TAKAGISHUICHI MACHIDA
著者情報
ジャーナル オープンアクセス

2018 年 64 巻 Suppl.1 号 p. 103-106

詳細
抄録

Objective: In response to both endogenous and exogenous stimuli, mammalian mitochondria undergo dynamic structural remodeling via fusion and fission events (mitochondrial dynamics), which support biogenesis, maintain functional integrity, and assist in the removal of dysfunctional organelles. However, whether the responses to high-fat diet change mitochondrial dynamics in both slow- and fast-twitch muscles remains unknown. The purpose of this study was to investigate the effects of a high-fat diet on the expression of mitochondrial fission-related protein Drp1 in both slow- and fast-twitch muscles.

Materials and Methods: Female Sprague-Dawley rats (4-weeks old) were assigned to either a high-fat diet or a sedentary control environment for 8 weeks. After 8 weeks of high-fat diet, the animals were sacrificed, and red and white portions of the gastrocnemius muscle were dissected. These muscles were prepared for western blotting analysis to quantify the protein level of Drp1, which regulates mitochondrial fission.

Results: After 8 weeks of feeding with high-fat diet, there were no changes in the levels of Drp1 in either slow- or fast-twitch muscles.

Conclusions: In conclusion, high-fat diet did not affect the protein level of Drp1 in the slow- or fast-twitch muscles.

著者関連情報
© 2018 The Juntendo Medical Society. This is an open access article distributed under the terms of Creative Commons Attribution License (CC BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original source is properly credited.

This article is licensed under a Creative Commons [Attribution 4.0 International] license.
https://creativecommons.org/licenses/by/4.0/
前の記事 次の記事
feedback
Top