順天堂醫事雑誌
Online ISSN : 2188-2126
Print ISSN : 2187-9737
ISSN-L : 2187-9737
Original Articles
Identification of MicroRNA 15b-3p as a Diagnostic Marker for Early Stage of Colorectal Cancer Through Comprehensive RNA Analysis
RYOICHI TSUKAMOTOMASAKI HOSOYAMIDORI FUKAYANORIHIKO YOKOYAMASHINGO KAWANOKIICHI SUGIMOTOMAKOTO TAKAHASHIKAZUHIRO SAKAMOTOHIROMITSU KOMIYAMA
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ジャーナル オープンアクセス

2021 年 67 巻 3 号 p. 272-281

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Objective Early detection is the key to treatment outcome in colorectal cancer (CRC). This study aimed to identify microRNAs (miRNAs) as a useful diagnostic marker for early-stage CRC.

Materials We selected 7 cases without lymph node metastasis or any typical risk factors from 52 stage II CRC cases. Blood sampled before and after surgery, and cancer and normal tissue samples of the selected cases were supplied. Aside from this, a public data set of miRNA expression in the serum of patients with CRC and healthy controls obtained from Gene Expression Omnibus National Center for Biotechnology Information (GSE40247) was used.

Methods A microarray analysis was conducted using the total RNA extracted from the serum in 4 cases. The expression levels of RNAs in the bloods of remaining 3 cases and cancer and normal tissues of all the 7 cases were evaluated using real-time quantitative polymerase chain reaction (RT-qPCR). Based on the public data, the expression level of miRNAs in the serum of patients with CRC was compared with those of healthy controls (HC).

Results miR-15b-3p and miR-339-5p were significantly decreased after the surgery (n=4, p<0.05). Higher expression in preoperative blood was verified for both miR15b-3p (p=0.040) and miR-339-5p (p=0.046) (n=3). The cancer tissue-specific higher expression was detected for miR-15b-3p (p=0.0490) but not for miR339-5p (p=0.095). In the public data, the expression levels of miR-15b-3p in stages I, II and IIIa CRC patients were higher than those in HC (p=0.041, 0.036, and 0.014, respectively). The expression levels of miR-339-5p were not different among CRC and HC sera (p>0.05).

Conclusions miR-15b-3p may be a useful diagnostic marker for early-stage CRC.

著者関連情報
© 2021 The Juntendo Medical Society. This is an open access article distributed under the terms of Creative Commons Attribution License (CC BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original source is properly credited.

This article is licensed under a Creative Commons [Attribution 4.0 International] license.
https://creativecommons.org/licenses/by/4.0/
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