Upstream Factors Regulating Maturation/M-phase Promoting Factor Activity during Oocyte Maturation

Kunihiko Naito; Yukio Nishimura; Tadashi Yamamuro; Takuma Shimaoka; Wataru Fujii; Mari Suzuki; Takanori Nishimura; Kiyoshi Kano

doi:10.1274/jmor.27.27

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Upstream Factors Regulating Maturation/M-phase Promoting Factor Activity during Oocyte Maturation

Kunihiko Naito ^†, Yukio Nishimura ^†, Tadashi Yamamuro ^†, Takuma Shimaoka ^†, Wataru Fujii ^†, Mari Suzuki ^†, Takanori Nishimura ^†, Kiyoshi Kano ^†

Author information

Kunihiko Naito ^†
Laboratory of Applied Genetics, Graduate School of Agriculture and Life Sciences, The University of Tokyo
Yukio Nishimura ^†
Laboratory of Applied Genetics, Graduate School of Agriculture and Life Sciences, The University of Tokyo
Tadashi Yamamuro ^†
Laboratory of Applied Genetics, Graduate School of Agriculture and Life Sciences, The University of Tokyo
Takuma Shimaoka ^†
Laboratory of Applied Genetics, Graduate School of Agriculture and Life Sciences, The University of Tokyo
Wataru Fujii ^†
Laboratory of Applied Genetics, Graduate School of Agriculture and Life Sciences, The University of Tokyo
Mari Suzuki ^†
Laboratory of Applied Genetics, Graduate School of Agriculture and Life Sciences, The University of Tokyo
Takanori Nishimura ^†
Laboratory of Applied Genetics, Graduate School of Agriculture and Life Sciences, The University of Tokyo
Kiyoshi Kano ^†
Laboratory of Applied Genetics, Graduate School of Agriculture and Life Sciences, The University of Tokyo
Author contributions

†These authors contributed equally: Kunihiko Naito, Yukio Nishimura, Tadashi Yamamuro, Takuma Shimaoka, Wataru Fujii, Mari Suzuki, Takanori Nishimura, Kiyoshi Kano

Keywords: MPF, Cyclin B, Cdc2, Pig

JOURNAL FREE ACCESS

2010 Volume 27 Issue 1 Pages 27-34

DOI https://doi.org/10.1274/jmor.27.27

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Abstract

Maturation/M-phase promoting factor (MPF) activity is regulated by the Cyclin B concentration and phosphorylation states of Cdc2 kinase. In the present review, we summarize the factors regulating the synthesis and degradation of Cyclin B and the phosphorylation and dephosphorylation of Cdc2. We focuse on Aurora A and cytoplasmic polyadenylation element binding protein (CPEB), which are involved in cytoplasmic polyadenylation, for Cyclin B synthesis; on anaphase-promoting complex (APC) activating factors, FZR1 and CDC20, for Cyclin B degradation; on Cdk7 and Wee1B for Cdc2 phosphorylation, and on Cdc25 for Cdc2 dephosphorylation. We describe our recent results concerning these factors in porcine oocytes, and discusse overall regulation of MPF activity during porcine oocyte maturation.

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