ウシカエルとその幼生 (Rana catesbeiana) の核蛋白質の比較

抄録

Chromatin is composed of DNA, RNA, histones and non-histone chromosomal proteins NHCP. Although there are several methods for the extraction of NHCP, it is difficult to isolate each of NHCP in its native form.
The author devised a simple electrophoretic method for the analysis of NHCP and used it to study the chromatin of the tadpole and bullfrog, Rana catesbeiana. The method was better in the reproduction and yield of proteins than previously used methods. Histones and NHCP were extracted from the chromatines of various tissues of the bullfrog and tadpole, and were analyzed by the electrophoretic method. No qualitative but quantitative variations were found in the composition of NHCP among the tissues examined. The tissues of the tadpole and frog were classified into three groups according to their electrophoretic patterns of NHCP; brain, heart muscle and spleen showing almost the same pattern, liver and kidney showing a slightly different pattern, and erythrocytes showing a markedly different pattern. Component D 3 oocurs in the frog liver, but not in the tadpole. An increase in the tadpole liver was found during natural and thyroxine-induced metamorphosis.