Journal of Nutritional Science and Vitaminology
Online ISSN : 1881-7742
Print ISSN : 0301-4800
ISSN-L : 0301-4800
Regular Paper
A Flow Injection Chemiluminescence Method for the Determination of Retinol in Pharmaceutical Formulations by Using Luminol-Diperiodatoargentate(III) Reaction
AMBARMuhammad ASGHARFuad AMEENSaleh AL-NADHARIAmir WASEEMMohammad YAQOOBAbdullah A. ALARFAJAbdul NABI
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2020 Volume 66 Issue 1 Pages 10-18

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Abstract

In this paper, a chemiluminescence (CL) method is proposed for retinol determination by combining flow injection (FI) methodology. The CL reaction is based on the oxidation of luminol by diperiodatoargentate(III) (DPA) in the presence of retinol. Under the optimum conditions, the relative CL intensity was linear to the concentration of retinol over the range 5.0×103-14 mg L1 (y=347.26x+2.5944, R2=0.9999, n=8) with limit of detection (LOD) of 1.5×103 mg L1 (S/N=3) and limit of quantification (LOQ) of 5.0×103 mg L1 (S/N=10). The relative standard deviation (RSD) was from 1.04-3.4% over the range studied and injection throughputs of 150 h1. The method was satisfactorily applied to retinol in pharmaceutical formulation samples. The samples were saponified and extracted with liquid-liquid extraction using ether as an extractant. The possible CL mechanism is supported by CL and UV-visible spectrophotometric studies.

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© 2020 by the Center for Academic Publications Japan
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