THIN LAYER CHROMATOGRAPHY OF VITAMIN B₁₂

Abstract

Several excellent solvents for the thin-layer chromatography (TLC) of vitamin B₁₂ and the related compounds were found out using Aluminium oxide G, Kieselgel G and Kieselguhr G as inorganic adsorbents, and P-cellulose as well as DEAF-cellulose as organic ones. Since there are many vitamin B₁₂ factors of similar Rf values, it is still difficult to separate each factor in the mixture completely and identify it. It is advisable to prepurify or fractionate prior to the TLC of vitamin B₁₂.