Journal of Nutritional Science and Vitaminology
Online ISSN : 1881-7742
Print ISSN : 0301-4800
ISSN-L : 0301-4800
SOME CHARACTERISTICS OF THE DIHYDROFOLATE SYNTHETASE FROM SERRATIA INDICA
Masamichi IKEDAKazuo IWAI
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1976 Volume 22 Issue 5 Pages 365-373

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Abstract

Dihydrofolate synthetase (EC 6. 3. 2. 12) from Serratia indica IFO 3759 require a divalent cation and a univalent cation for its ac tivity. The divalent cation requirement was satisfied by magnesium ion, manganese ion or ferrous ion. High activity was obtained with 5 mM of magnesium ion. The effect of manganese ion was weak. The univalent cation requirement was satisfied by potassium ion, ammonium ion or rubidium ion, and high activity was obtained with 100 mM of each univalent cation. Increase in the potassium concentration lowered Km values for dihydropteroate and L-glutamate, and raised Vmax for ATP and dihydropteroate. Potassium ion had little effect on Km value for ATP. These results suggest that potassium ion may function on the affinities of dihydropteroate and L-glutamate to the enzyme.
Dihydrofolate synthetase was inhibited by the addition of reduced forms of homopteroic acid. Stronger inhibition was observed by dihydrohomopteroate than by tetrahydrohomopteroate.

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