Journal of Nutritional Science and Vitaminology
Online ISSN : 1881-7742
Print ISSN : 0301-4800
ISSN-L : 0301-4800
IDENTIFICATION OF THE SECOND PRODUCT OF THE RIBOFLAVIN SYNTHETASE REACTION
Hisateru MITSUDATomonori NADAMOTOKenji NAKAJIMAI
Author information
JOURNALS FREE ACCESS

1976 Volume 22 Issue 5 Pages 381-387

Details
Abstract

This study was concerned with the detailed identification of the second product involved in the riboflavin synthetase reaction with riboflavin synthetase from Eremothecium ashbyii and a trapping agent, glyoxal. Thus, a green fluorescent compound accumulated during the incubation. The compound was purified through various column chromatography steps, and was examined by UV, IR, excitation and emission spectra and paper chromatography to prove that the isolated compound was 8-ribityllumazine. Accordingly it was concluded that a second product in riboflavin synthetase reaction was 4-ribitylamino-5amino-2, 6-dihydroxypyrimidine, the fragment, except for C-6 and C-7 of 8-ribityllumazine, being an incorporated glyoxal portion.

Information related to the author
© the Center for Academic Publications Japan
Previous article Next article
feedback
Top