PROTEINS OF CHICK DUODENAL BRUSH BORDERS DURING DEVELOPMENTAL CHANGES

Abstract

Duodenal brush border membrane proteins were studied in chicks at different developmental stages. The protein pattern obtained from polyacrylamide gels with 2-day-old chick preparations was dis tinctly different from that obtained with 20-day embryos. The most remarkable changes were seen in the region of a protein with an Rf of 0.25, an area with high sucrase and maltase activity, and in the region of a protein with an Rf of 0.28, which was characterized by alkaline phosphatase activity. These proteins reacted strongly with carbohy drate stain after hatching.