Journal of Nutritional Science and Vitaminology
Online ISSN : 1881-7742
Print ISSN : 0301-4800
ISSN-L : 0301-4800
λDNA-Fragmenting Actions of Ascorbic Acid and Triose Reductone in the Presence of Cu2+
Kazuki SHINOHARAMasayasu SOMichiko NONAKAKazuo NISHIYAMAHiroki MURAKAMIHirohisa OMURAI
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1983 Volume 29 Issue 6 Pages 671-678

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Abstract

Lambda DNA-fragmenting actions of ascorbic acid (AsA) and triose reductone (TR) in the presence of Cu2+ were studied. The mixture of AsA or TR and Cu2+ caused a marked fragmentation ofλ, DNA (3.2×107 daltons) within the first 1 min of reaction. Further incubation resulted in accumulation of the most abundant species of fragmented λDNA having a molecular weight of 1.3×105 daltons. The mixture of AsA or TR and Cu2+ fragmented calf thymus DNA to produce the fragmented DNA of which 5'-OH terminal groups have a mixture of free OH groups and phosphodiester linkage. The mixture of AsA or TR and Cu2+ was also found to fragment, DNA to produce dCMP predominantly as 5'-OH terminal nucleotides.

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