Effects of Active Oxygen Scavengers on the Peroxidation of Linoleic Acid Catalyzed by Dehydro-L-Ascorbic Acid or Its Degradation Products

Abstract

The addition of 1, 4-diazabicyclo-[2, 2, 2] octane (DABCO) (100mM) or 1, 2, -dihydroxybenzene-3, 5-disulfonic acid (Tiron) (1mM) to a reaction mixture containing 10mM linoleic acid (LA), 20% EtOH, and 135μM dehydroascorbic acid (DHA) as a catalyst suppressed LA per-oxidation, but the addition of mannitol (100mM), uric acid (100μM), and catalase (6.5 units) did not. DHA or 2, 3-diketo-L-gulonic acid (DKG) accelerated LA peroxidation, but the splitting products of DHA did not affect LA peroxidation. These results suggest that some specific radicals are liberated in the degradation of DHA or DKG.