The Effects of the Preincubation with Vitamin B2-Acid on Ferredoxin-NADP+ Reductase

Minoru KATO; Sei TACHIBANA

doi:10.3177/jnsv.36.431

Journal of Nutritional Science and Vitaminology

Online ISSN : 1881-7742
Print ISSN : 0301-4800
ISSN-L : 0301-4800

The Effects of the Preincubation with Vitamin B₂-Acid on Ferredoxin-NADP⁺ Reductase

Minoru KATO, Sei TACHIBANA

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Keywords: vitamin B₂-acid, ferredoxin-NADP₊ reductase, cytochrome c reductase activity, diaphorase activity, Chattonella antiqua, kinetic parameter

JOURNAL FREE ACCESS

1990 Volume 36 Issue 5 Pages 431-436

DOI https://doi.org/10.3177/jnsv.36.431

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Abstract

The effects of vitamin B₂-acid on both cytochrome c red-uctase and diaphorase activities of ferredoxin-NADP₊ reductase [EC 1.18.1.2] were investigated with enzyme kinetics. Vitamin B₂-acid was shown to serve as an electron carrier for the two activities, as well as riboflavin or flavin mononucleotide (FMN). The two activities, however, were irreversibly affected by the preincubation of the enzyme with vitamin B₂-acid under certain conditions, while riboflavin or FMN did not show such effects.

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