Journal of Nutritional Science and Vitaminology
Online ISSN : 1881-7742
Print ISSN : 0301-4800
ISSN-L : 0301-4800
Simultaneous Determination of Hydrogen, Methane and Carbon Dioxide of Breath Using Gas-Solid Chromatography
Keisuke TSUJIMasatoshi SHIMIZUYoshinori NISHIMURAYasue NAKAGAWATomio ICHIKAWA
Author information
JOURNALS FREE ACCESS

1992 Volume 38 Issue 1 Pages 103-109

Details
Abstract

The analysis of respiratory hydrogen and methane was estimated as a useful index of intestinal fermentation of undigestible carbohydrate. A simultaneous and precious analysis of these gases as well as carbon dioxide was studied. A gas-impermeable multi-laminated film bag metalized by aluminum vapor was fitted to use as a storage bag; its impermeability was verified by measuring the residual rate of hydrogen after 3 months' storage. Hydrogen, methane and carbon dioxide of the breath gas even at 1ppm could be determined simultaneously by using a gas-solid chromatography installed with a photoionization detector and active carbon column. To observe the genesis of hydrogen and methane after carbohydrate ingestion, pectin, a typical water-soluble dietary fiber, was fed to healthy volunteers. Increasing excretion of pulmonary hydrogen or methane showed the sign of intestinal fermentation as the results of carbohydrate malabsorption.

Information related to the author
© the Center for Academic Publications Japan
Previous article
feedback
Top