Pattern of Fecal Endogenous Nitrogen Excretion in Rats Fed Leguminous Diets

Abstract

The main objective of this investigation was to establish the pattern in relation to time of the rat fecal endogenous nitrogen excretion by continuous feeding of balanced diets containing common peas, cowpeas or common beans as the protein sources (10% protein), labeled with 1.000 atoms % of ¹⁵N-excess. Nitrogen of endogenous origin was measured by the isotope dilution method in a 6-day experiment. Fecal excretion of endogenous nitrogen of rats fed the leguminous diets was roughly twice that of rats fed the non-protein diet (88mg, 42mg), and the excretion of total fecal nitrogen did not differ among leguminous diets. From the third to the sixth day of the experiment, the endogenous nitrogen excretion, either as a percentage or quantity (mg), attained a statistically non different value (p>0.05). A common pattern of excretion of fecal endogenous nitrogen as a function of time was expressed by a strongly negative (r<-0.95) power regression (y=A⋅x^-B) for the common pea, the cowpea or the common bean diets. Conversely, the excretion of dietary nitrogen did not show a common pattern as a function of time for all experimental diets.