Journal of Nutritional Science and Vitaminology
Online ISSN : 1881-7742
Print ISSN : 0301-4800
ISSN-L : 0301-4800
Effects of Fatty Acids on Accumulation and Secretion of Histamine in RBL-2H3 Cells and Leukotriene Release from Peritoneal Exudate Cells Isolated from Wistar Rats
Koji YAMADAMitsuo MORINoritaka MATSUOKentaro SHOJITakashi UEYAMAMichihiro SUGANO
Author information

1996 Volume 42 Issue 4 Pages 301-311


Effects of fatty acids on accumulation and secretion of histamine in rat basophilic leukemia RBL-2H3 cells and leukotriene release from peritoneal exudate cells isolated from Wistar rats were examined in relation to the manifestation of type I allergic reactions. When RBL-2H3 cells were cultured for 24h in the presence of 1 mM short chain fatty acids, a marked increase in histamine accumulation was observed, especially with butyric acid. In addition, Ca-ionophore A23187-stimulated histamine release was enhanced in the cells treated with 0.1mM mono to hexa unsaturated fatty acids with 18 to 22 carbonchains. On the other hand, LTB4 release from rat peritoneal exudate cells was inhibited in the presence of polyunsaturated fatty acids, both n-6 and n-3, having more than 3 double bonds. Inhibitory activity was enhanced by an increase in the number of double bonds, and docosahexaenoic acid (DHA) exerted the highest activity with complete inhibition at 0.1mM and 3 5.5% inhibition even at 10μM. A hydrophobic radical scavenger (9, 10-diphenylanthracene) and two antioxidants (butyrated hydroxytolu-ene and α-tocopherol) inhibited the production of LTB4, but hydrophilic counterparts (mannitol and ascorbic acid) did not. These results suggest that lipophilic anti-oxidative agents, as well as PUFA, inhibit the production of LTB4.

Information related to the author
© the Center for Academic Publications Japan
Previous article Next article