Journal of Nutritional Science and Vitaminology
Online ISSN : 1881-7742
Print ISSN : 0301-4800
ISSN-L : 0301-4800
The Assay of Ascorbic Acid in Serum Is Not Affected by Physiological Concentrations of Transferrin and Hemoglobin
Hiroshi IHARAYoshio SHINONaotaka HASHIZUMEAkiko HIRANOMitsumasa OKADA
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2003 Volume 49 Issue 4 Pages 289-291

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Abstract

Transferrin and hemoglobin have been reported to oxidize L-ascorbic acid (AA) in vitro. The aim of this study was to determine whether or not physiological concentrations of serum transferrin (reference range 22-45μmol/L) and hemoglobin (reference range 0-3.0μmol/L) interfer with the measurement of AA in the serum. Transferrin (33 to 41μmol/L) and hemoglobin (1.9μmollL) added to freshly pooled serum significantly decreased measured AA in the serum (p<0.05). However, we found that the magnitude of the decrease in AA due to transferrin at concentrations within the reference range or up to 80μmol/L was inconsequential, and had no clinical importance in diagnosing a low AA concentration. Hemoglobin at concentrations within the reference range had little affect on the serum AA measurement. However, when serum specimens were stored at 4°C for more than 1.5h, the magnitude of the decrease in AA due to hemoglobin at physiological concentrations may cause a misleading clinical diagnostic evaluation of low AA concentration.

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