Effect of nano modified titanium surface on adsorption of rat periodontal ligament cells

抄録

When natural teeth are lost, endosseous implants anchored via intimate bone­-to­-implant contacts are considered an appropriate replacement. The interactions between implants and host tissues depend on several factors. In particular, a growing body of evidence has demonstrated that the surface texture of an implant influences the response of the surrounding cells. Recent studies have shown that treating titanium with aqueous NaOH produces a nanostructured surface texture termed a titanium nanosheet (TNS). Furthermore, it was recently reported that a TNS surface promoted the osteogenic differentiation of rat bone marrow cells. We investigated whether a TNS surface may improve wound healing around endosseous implants. To test this, we assessed the effects of a TNS sur­face on the adhesion and differentiation of rat periodontal ligament (RPL) cells in vitro. The results demonstrated that RPL cells cultured on a TNS surface showed more adhe­sion and higher levels of osteogenic differentiation markers (Runx2 expression, alkaline phosphatase activity, calcium deposition, and osteocalcin secretion) than cells cultured on unmodified titanium. These findings indicate that RPL cells have the potential for in­creased bone formation on a TNS surface. Thus, TNS surface modification may warrant consideration for use in endosseous dental implants. (J Osaka Dent Univ 2018; 52: 37­-44)