オゾンによるタンパク変性効果,特に卵白アルブミンの抗原性獲得についての研究
1988 年 100 巻 1-2 号 p. 1-7
詳細
1988 年 100 巻 1-2 号 p. 1-7
Crystalline egg albumin denatured by exposure to ozone was analyzed by ultraviolet spectrophotometry. Rabbits were injected with denatured egg albumin to induce specific antibody. Native egg albumin (NEa) and ozone denatured egg albumin (O3Ea) cross-reacted with antinative egg albumin antibody (anti-N) and anti-ozone denatured egg albimin antibody (anti-O3), respectively. Heat denatured egg albumin (HEa) and O3Ea cross-reacted with anti-heat denatured antibody (anti-H). The precipitin lines in immuno-double diffusion tests between NEa and anti-N and between O3Ea and anti-N were fused with spur formation. The precipitin lines of O3Ea with anti-O3 and HEa with anti-O3 were fused with slight spur formation. Two precipitin lines of O3Ea with anti-O3 were observed by immunoelectrophoresis. The faster line had similar majority to the line of NEa and anti-N. The other line had a slightly slower mobility than the former line, indicating that this slower line was due to ozone specific antigen.
