岡山医学会雑誌
Online ISSN : 1882-4528
Print ISSN : 0030-1558
蛍光色素遊出法を応用した細菌脂質膜安定性の研究
長町 榮子
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ジャーナル フリー

1988 年 100 巻 1-2 号 p. 107-117

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I have developed a method for measuring the anti-osmotic stability of liposomes prepared with lipids extracted from bacteria, using the release of carboxyfluorescein trapped in liposomes as an indicator. I also examined the sub-second physical changes of liposomes submerged in a solution of low osmotic pressure with a stopped flow spectrophotometer. The trapped carboxyfluorescein was released when the liposomes burst upon the inflow of excess water. Liposomes prepared with the lipids of a stable S. aureus L-form strain or Mycoplasma orale were more resistant to osmotic pressure than those prepared from the wild strain of S. aureus. It was found that cardiolipin enhanced the membrane-stability in S. aureus and cholesterol (about 30% of the total membrane lipids) in Mycoplasma orale. By the present method, the resistibility of membranes to low osmotic pressure could be determined precisely with extracted and purified bacterial membrane lipids.

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