骨髓穿刺に関する基礎的研究

第1編 骨髓血管の破壞及び再生に関する研究

抄録

Employing 99 rabbits, white and male, in size about 2kg., conducted a femur marrow puncture, and by pouring Indeanink and Berliner blue into a femur marrow vessel, immediately after the operation until 100th day, made some transparent specimens, casts of vessels by means of injection of Resin, as well as H. E. sections for the purpose of investigating the regenerative process of the bone-marrow, especially to know the unique property of sines nets of the marrow; results were as follows: Further, I studied on destruction and regeneration of 3 corpses that had underwent a sternal puncture before death.
(1) After the puncture. that part remains, the cavity of which has been constructed owing to the destruction, filled with blood; yet it happens seldom that such thick vessels as the principal artery or principal sinus should be broken.
(2) The regeneration of sinus nets, begins on 3rd day, roundabout the destructed part; the invasion of regenerative vessels into a part of destruction becomes gradually strong from about 4th day.
Moreover, their regenerative conditions look like the sporting or budding of trees, and seen to formulate come sinus nets by anastmosis of one regenerative sinus with another, or a regenertive sinus and completed sinus.
(3) Though small in number. the regeneration of arterial capillaries are seen in the destructed part on about 4th day of operation, which gives birth to anastmosis with some regenerative sinus nets in the neighbourhood, or already completed ones.
But no anastomosis between arterial capillaries could be detected.
(4) It took about 7-12 days for an unfinished atypical sinus nets that were thin and variously-formed to cover the whole destructed part.
(5) Most of the regenerative marrow vessels near the destructed part of the bone were derived from those vessles due to endoperiost, and due to anastomosis with regenerative vessels in the bone marrow.
(6) The so-called intersinusoidal capillaries are seen not only under the feeble state of bone-marrow function, but also in the regenerative process of the bone-marrow vessels.
(7) The regenerative faculty, or the regenerative function of bone marrow vessels mainly depends upon age of those test rabbits; it has proved much stronger in young rabbits than in old ones.
(8) In case the marrow-punture is done continually for a short time, clinically, care should be taken to except at least over 20 mm distant place from the previpus one.