1958 年 70 巻 4 号 p. 1099-1102
In the determination of contents of ammonia and glutamine in the brains of normal rats and mice by Conway's method of micro-diffusion analysis, the author obtained the following results.
1) In the case of the normal mouse brain frozen and fixed in liquid nitrogen, the brain ammonia was 0.57 mg.%.
2) In the case of the normal rat brain extracted by opening the skull and frozen with dry ice acetone, the brain ammonia was 0.62 mg.%, while the brain glutaminewas 80.3 mg.%.
3) In the case of normal rat decapitated and frozen in liquid nitrogen, the brain ammonia was 1.80 mg.%, showing a quite high value.
4) In the the case where the rat, which had been exhaused by making it swim in water, and frozen in liquid nitrogen, the brain ammonia was 0.91 mg.%, showing no increase over that of the control