1959 年 71 巻 3-1 号 p. 1099-1111
The author studied the influence of ultra-violet irradiation on R. tsutsugamushi which had been isolated from the patients of Umayado disease in Kagawa Prefecture. The liver and spleen emulsions of mice infected with the human strain (Mitani) and the rat strain (XXII) were irradiated with ultra-violet ray. The results are briefly summarized as follows:
1) Ultra-violet irradiation for 60 seconds was not sufficient for inactivation of rickettsiae, but that for 120 to 160 seconds inactivated rickettsiae nearly completely.
2) The infectious protection index by thus inactivated rickettsiae was below 1.5, and the immune effect was low and indefinite; this is considered due to the low and indefinite quantity of rickettsiae in the liver and spleen emulsion. From this fact and the results of the experiments with Formalin-inactivated vaccine, it was inferred that ultra-violet irradiation has some effect on rickettsiae themselves.
3) For dilution of rickettsial emulsion, PGS (Snyder), 30% sucrose and saline (pH 7.6) solutions were used, but the sorts of solutions had no noticeable influence on the inactivation time by ultra-violet irradiation or on the antigenicity of the inactivated one.
4) The immune effect by inoculation of thus inactivated emulsion was determined by log LD50 in mice and that in the controle group. The fatal cases meant the infection of rickettsiae, but, even in non-fatal cases, the infection of rickettsiae was proved by staining and serial passage.