人癌及びマウス癌組織の組織培養に関する研究

第I編 人子宮頸癌,人淋巴腺転移癌,人上顎洞癌, Bashford氏マウス癌及び正常マウス肝組織の組織培養並びに培養液の水素イオン濃度の変化に就いて

抄録

In the present experiment, tissue cultures of human cervical carcinoma, human metastasized carcinoma in lymph node, human upper jaw carcinoma, Bashford's mouse carcinoma, and normal mouse liver tissue were performed by using roller-tube method. The tissue growth patterns, cell counts and relative growth rate, hydrogen ion concentration in culture media, and histological examination of these tissues in culture were observed. The following are the results.
1. Epidermoid carcinoma of human cervix, rquamous cell carcinoma of human upper jaw, carcinoma simplex in metastasized lymph node, Bashford's mouse carcinoma, and normal mouse liver tissue were cultivated in the media composed of desiccated human plasma solution, chick embryo extract, and Hanks' balanced salt solution by using the roller-tube method. Epidermoid carcinoma of human cervix was successively maintained for 4 months, squamous cell carcinoma of human upper jaw and carcinoma simplex in metastasized lymph node were maintained for 2 weeks and 2 months respectively. Bashford's mouse carcinoma and normal mouse liver tissue were maintained for 1 month and 1 week respectively.
2. The tissue growth patterns of human and animal tumors, and normal mouse liver tissue showed the membraneous sheet formation like a halo. However, 3-4 weeks after tissue culture many small cell groups appeared and they were connected with each other. And this tissue growth pattern showed no difference between malignant and normal tissues.
3. Cell counts were taken in the culture of epidermoid carcinoma of human cervix, Bashford's mouse carcinoma and normal mouse liver tissue. The number of cells increased rapidly in malignant tissues when compared with that of normal mouse liver tissue. Mauy mitosis could be seen in the tissue cultures of squamous cell carcinoma and carcinoma simplex, but their cell counts were not carried out.
4. The pH changes in tissue culture media was estimated. The rapid increases of hydrogen ion concentration in the course of tissue culture was proportional to the cell increase rate, and this is one of the characteristics of malignant tissues in vitro.
5. The histological examination of the cells derived from tissue culture and original tissues was carried out. Atypical large polynuclear cells generating in the tissue culture of carcinoma simplex could be considered as representing the malignant trasformation. And abundant mitosis, anaplastic change, and rapid cell increase were used as the criteria for the evaluation of malignancy in every tissue cells derived from both normal and malignant tissues in vitro.