肝臓組織培養に関する研究
第2編 家兎肝臓の廻転培養法による増生様式並びに出現する諸細胞に関する観察
1963 年 75 巻 11-12 号 p. 991-1000
詳細
1963 年 75 巻 11-12 号 p. 991-1000
By means of roller tube culture of rabbit liver the author studied the tissue growth pattern and various cells appearing in the growth area during the culture.
1) One to 2 days after the start of culture wandering cells and fibroblasts grow and wander out from the explants, and then from the 3 rd day or thereabout liver cells grow. The tissue growth continues up to the 6 th day, but therafter cells begin to degenerate unless the medium is replaced with a fresh one.
2) By the degree of the growth of liver cells and fibroblasts that appear in the growth area, the growth pattern can be classified in A and B type. A consists mainly of liver cells, dividing into 2 subtypes furthermore, and B type consists mainly of fibroblasts, dividing into 5 subtypes.
3) By tissue culture the number of binucleated liver cells decrease to 1/41/5 of number of normal rabbit on 4 th day, and increase gradually followed the duration of culture. On 10th day the number of binucleated cells recover to 1/3 of normal rabbit. The decrease of the number of binucleated liver cells indicates the accerelation of growth or regeneration of liver cells.
