腫瘍膜蛋白の抽出および免疫学的指標としての抽出蛋白による皮内反応
第1編 DOCおよび3 MKCL抽出膜蛋白によるマクロファージ遊走阻止活性の比較-特に抽出方法の違いによるマクロファージ遊走阻止活性の差を中心として-
キーワード:
エールリッヒ腹水癌細胞,
MH 134細胞
1983 年 95 巻 3-4 号 p. 185-193
詳細
1983 年 95 巻 3-4 号 p. 185-193
Ehrlich ascites cancer cells and MHl34 cells were extracted with Deoxycholate(DOC) or 3MKCL. The antigenicity of these extracts was studied with the macrophage migration inhibitory test (MI-test), and the results can be presented briefly as follows:
1. The extract from DOC solubilized Ehrlich ascites cancer cells had about 50 times the antigenic titer of the extract from 3MKCL solubilized cells.
2. The extract from DOC solubilized MHl34 cells had an antigenic effect at 500ug/ml but the extract from 3MKCL solubilized cells had no effect at any concentration.
3. These extracts were fractionated with Sephadex G-200 and the antigenic sites were determined with the MI-test.
