This paper describes a method for the isolation of lymphocytes from monkey (M. irus (g)) blood.
Monkey blood (20ml) drawn into a sterile vacutainer (Terumo Venojec System, Tokyo) containing preservative-free heparin (20units) was diluted with an equal volume of PBS (pH 7.2). The solution was carefully layered on the top of the 29: 10 (v/v) mixture (3ml, S. G. 1.072) of 8.5% ficoll solution and 33.33% isopaque solution and centrifuged at 2300 r. p. m. for 15min. at room temperature. Purity of the lymphocytes thus obtained was 89.8% and the recovery was 80.52%. They were consisted of small lymphocytes, (84.8%), medium lymphocytes (13.4%), and large lymphocytes (2.2%). The survival rate of lymphocytes immediately after isolation was more than 98%. The monkey lymphocytes could not be separated by the ficoll-isopaque solution (S. G. 1.076-1.078) generally used for the isolation of human lymphocytes.