唾液腺におけるマウスサイトメガロウイルスの潜伏感染に関する研究
1986 年 28 巻 5 号 p. 620-632
詳細
1986 年 28 巻 5 号 p. 620-632
Mouse cytomegalovirus (MCMV) infected cells were investigated for analysis of MCMVlatent infection and reactivation of MCMV in vivo. BALB/c mice were infected ip with 2×105.0 PFU of MCMV (Smith strain), which was grown in BALB/c mouse embryo cells (MEC) and the infectivities in the spleen, liver, pancreas and salivary gland were assayed with MEC after various days of in-fection. MCMV could be detected temporarily in the spleen and liver for 3-5 days after i nfection but MCMV could be recognized after 8 weeks in the pancreas. In the case of the salivary gland, MCMV could be detected 28 weeks after infection. On the other hand, neutralizing antibody in the sera appeared from 2 weeks after the infection and MCMV infectivity in the pancreas was gradually extinguished with the increase of antibody titer but that in the salivary gland was not.
When thin-sections of salivary glands were stained with hematoxylin -eosin, about 91% of giantcells were observed in the intercalated duct of salivary gland and furthermore the MCMV anti gen wasproved to be present in the giant cells by indirect immunofluorescent antibody staining.
When mice were administered with cyclophosphamide (CY) aft er 10 weeks of MCMV infection, the number of suppressor T cells, helper T cells, natural kill er cells and B cells in splenocytes was extremely reduced and the giant cells appeared again in the interc alated duct of the salivary gland.
These results clearly indicated that MCMV can infect specifically the intercalated duct of the salivary gland and could change to a persistent and then a latent state in the intercalated duct cells.
