Supercritical carbon dioxide (SC-CO2) is a suitable substance to extract non-polar substances (triacylglycerols). However it has not proven effective in the extraction of polar substances. The efficient use of SC-CO2 and ethanol mixture to extract and fractionate phospholipids from salmon fish roe was therefore investigated. Extraction was performed at low pressure and temperature (17.7 MPa and 33°C) to avoid oxidation of polyunsaturated fatty acids. Phospholipids were not found to be extracted with 0- and 5%-ethanol in SC-CO2. However extractions with 10, 15 or 20%-ethanol in SC-CO2 were effective in extracting phospholipids. The amount of extracted phospholipids increased with increased addition of ethanol. When the extraction was performed with SC-CO2 and 20%-ethanol mixture, more than 80% of the phospholipids were recovered.
2004 by Japan Oil Chemists' Society