Comparative Study of the Effect of Basal Diet Formulation, Dietary Fat and Cholesterol Levels on the Development of Aortic Atherosclerotic Lesions in B6.KOR-Apoeshl Mice

Takafumi Okabe; Takayoshi Toda; Natthanan Nukitrangsan; Masashi Inafuku; Hironori Iwasaki; Teruyoshi Yanagita; Hirosuke Oku

doi:10.5650/jos.59.161

Oils and Fats

Comparative Study of the Effect of Basal Diet Formulation, Dietary Fat and Cholesterol Levels on the Development of Aortic Atherosclerotic Lesions in B6.KOR-Apoe^shl Mice

Takafumi Okabe, Takayoshi Toda, Natthanan Nukitrangsan, Masashi Inafuku, Hironori Iwasaki, Teruyoshi Yanagita, Hirosuke Oku

Author information

Takafumi Okabe
United Graduate School of Agricultural Sciences, Kagoshima University
Center of Molecular Biosciences, Tropical Biosphere Research Center, University of the Ryukyus
Takayoshi Toda
Department of Clinical Laboratory Medicine, School of Medicine, University of the Ryukyus Hospital
Natthanan Nukitrangsan
United Graduate School of Agricultural Sciences, Kagoshima University
Center of Molecular Biosciences, Tropical Biosphere Research Center, University of the Ryukyus
Masashi Inafuku
Transdisciplinary Research Organization for Subtropical Island Studies, University of the Ryukyus
Hironori Iwasaki
Center of Molecular Biosciences, Tropical Biosphere Research Center, University of the Ryukyus
Teruyoshi Yanagita
Laboratory of Nutrition Biochemistry, Department of Applied Biochemistry and Food Science, Saga University
Hirosuke Oku
Center of Molecular Biosciences, Tropical Biosphere Research Center, University of the Ryukyus

Keywords: B6.KOR-Apoe^shl mice, atherosclerosis, cholesterol, AIN diets, oil

JOURNAL FREE ACCESS

2010 Volume 59 Issue 4 Pages 161-167

DOI https://doi.org/10.5650/jos.59.161

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Abstract

To optimize the adequate atherogenic diet composition for nutritional atherosclerosis studies utilizing B6.KOR-Apoe^shl mice, we investigated the effect of dietary cholesterol, AIN formula, and dietary fats on the development of atherosclerosis. Cholesterol supplementation (0.15-2%) for 12 weeks resulted in a dose-dependent increase in the development of atherosclerosis. Furthermore, there were no significant differences in the degree of atherosclerosis between B6.KOR-Apoe^shl mice fed a modified-AIN-76™ diet and those fed a modified-AIN-93M diet containing corn oil or soybean oil for 10 and 12 weeks. The present experiment indicates that the adequate dietary level of cholesterol was 0.15%, and that further studies are necessary to determine the optimal level of various types of dietary oils for nutritional atherosclerosis experiments in B6.KOR-Apoe^shl mice.

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