Abstract
In the previous paper, the authors reported that more than 55% of the toxic fraction separated from thermally oxidized oil was absorbed through the intestine of rats. The present study is for investigation of the structure and the behavior of this toxic fraction in rats.
The toxic fraction prepared from the thermally oxidized soybean oil by chromatography on silicic acid column as described previously, was fed to rats for 2 weeks. The feces were collected, and the fecal lipids were extracted and fractionated by column chromatography. For comparison of the toxicity, each fraction thus obtained and the toxic fraction of original polymerized oils were orally administered to mice.
The toxic components derived from the fecal lipids which were eluted into the diethyl ether fracion from silicic acid column, were comparable to dimer of triglycerides in molecular weight, and more toxic than that of the toxic fraction of original polymerized oil. Chromatographic behavior, molecular weight, and the toxicity of the fecal lipids seem to indicate that at least a part of toxic components in the thermally oxidized oil undergoes little change in the body of rat and is excreted in the feces.
