子牛胸腺のオクタデセン酸

精製と構造研究および抗腫瘍活性

抄録

A lipid fraction having an inhibitory activity against Ehrlich mouse ascites carcinoma was isolated from calf thymus. Thymus tissue was hydrolyzed under N₂ with 20% NaOH and the active material was extracted with ether after the hydrolysate was acidified with HCI. The crude material thus obtained was purified by the difference in solubility of its metal salts in organic solvents.
The growth of the tumor in mice was inhibited by the administration of either the crude material or the purified one in a dose of 5 mg/mouse/day or 2 mg/mouse/day respectively for 10 days. Further purification was subjected to a silica gel column chromatography as the Hg-complex of an active lipid fraction, and the chemical analyses were made on the fractions with TLC, GLC. and IR. The results indicated that the main component of the most purified fraction was a cis-octadecenoic acid.
This fatty acid was similar to oleic acid but it was far more active than oleic acid against Ehrlich ascites carcinoma. The GLC-MS and the result of oxidative cleavage suggested that the principle of the anti-tumor substance from calf thymus might be cis-Δ¹⁰- or cis-Δ¹¹-octadecenoic acid differing-from oleic acid in the location of the double bond.