GPC-HPLCを用いた生産管理のための飽和アシルグリセリンの迅速定量法
1987 年 36 巻 2 号 p. 120-124
詳細
1987 年 36 巻 2 号 p. 120-124
High performance liquid chromatography (HPLC) using gel permeation column was developed for the quantitative determination of saturated acylglycerols. It was possible to separate acylglycerols into their respective homologs within 20 min using Shim-pack HSG-15 H column (30 cm × 7.9 mm i.d.) under the following conditions : mobile phase, tetrahydrofuran; flow rate, 0.5mL/min; detector, refractive index detector. Mono-, di- and triacylglycerols in saturated acylglycerols were determined simultaneously using hexastearoyldipentaerythritol (HSDP) as the internal standard. This method had excellent reproducibility and was highly accurate. The determination of free glycerol and a relative response factor (RRF) method are also described. The present HPLC method was found quite very useful for the quality and process control of acylglycerol emulsifiers.
