糸状菌による脂質の生産に関する研究 (第22報)
Mortierella属菌によるγ-リノレン酸の生産
1989 年 38 巻 12 号 p. 1007-1015
詳細
1989 年 38 巻 12 号 p. 1007-1015
Nine fungi of Mortierella genus were cultivated batchwise in a 30L-fermenter in high con-centration glucose medium (100-200g/L). As a result, four strains attained a γ-linolenic acid (GLA) productivity exceeding 2g/L. The strain content of GLA in the fatty acids of the lipids obtained was from 6.6-7.8%. Using M. ramanniana var. angulispora IFO 8187, one of the four strains, the effects of various cultivation conditions on GLA productivity were investigated. Culti-vation at a higher content of GLA in the total lipids was generally noted in strains with lower lipid productivity, thus giving lower GLA yield. Consequently, cultivation to obtain high GLA yield is possible in a cultivation of high lipid productivity rather than high GLA content. Two types of repeated batch cultures with one and two fermenters, respectively, were made to obtain higher lipid productivity. A maximum lipid productivity of 13.9g/L/d was realized in the 2nd fermenter with a double stage repeated batch culture. GLA yield in the repeated batch culture increased with lipid productivity.
