遠心液-液クロマトグラフィーによるアシルグリセリンの分画 (第2報)
リパーゼ加水分解魚油からのEPAと DHA の濃縮
1992 年 41 巻 4 号 p. 312-316
詳細
1992 年 41 巻 4 号 p. 312-316
Hydrolyzed mixtures, derived from fish oil and containing free fatty acid, monoglyceride, diglyceride, and triglyceride, with hydrolysis rates about 30, 45, 60, 75 %, respectively, were applied to CPC to concentrate polyunsaturated fatty acids. Two solvents, n-hexane and acetonitrile, were used to fractionate hydrolyzed mixtures by CPC. In each case, the recovery of fractionated components exceeded 90%. In some reversed descending fractions, over 80 % of the fatty acid component consisted of icosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). More than 90 % of EPA and DHA was recovered in the reversed descending fractions. The concentrations of EPA and DHA in the reversed descending fractions increased with hydrolysis rate.
