Abstract
A sensitive HPLC method was developed for determing acylated sterylglycosides (ASG) in vegetable oil. ASG was concentrated by a silicagel cartridge and subsequently isolated by TLC from other glycosides. Derivatization of ASG with 1-anthroylnitrile was then carried out. Derivatized ASG was separated with HPLC in accordance with carbon number and degree of unsaturation of acyl and steryl groups. Data on an acyl/steryl combination of ASG species were obtained. ASG isolated from commercial soybean phospholipid was used as the standard substance. This method was applicable to extracts from oil seeds with polar solvent mixture. The limit of detection was about 5 ppm for edible oil.
