光散乱検出器を用いた HPLC によるトリアシルグリセリンの定量分析 (第2報)
補正法の検討
1994 年 43 巻 1 号 p. 51-56
詳細
1994 年 43 巻 1 号 p. 51-56
A reversed phase high performance liquid chromatography (HPLC) system with a light scattering detector (mass detector) was used for the quantitative analysis of triacylglycerol, and a correction method for the quantitative data was developed. Three eluting solvents acetinitrile : acetone = 65 : 35 (same as previous paper), acetonitrile dichloromethane = 75 : 25 and acetone were used for this purpose. Signal intensity from the detector was found to vary with the molecular structure of triacylglycerol and the solvent used, and to increase with sample retention time. Signal intensity was strongly influenced by retention time than was molecular structure. The relationship between signal intensity and injected sample amount could be expressed by an empirical equation. Two constants in the equation were datermined based on sample retention times. Quantitative data could thus be corrected by the equation proposed, signal intensity and retention time. The application of this method to some randomized vegetable oils indicated the corrected value to be more accurate than obtained by a refractive index detector.
