高速液体クロマトグラフィーによるサケ精巣のフラン酸の分析

抄録

The compositions of furan fatty acids (F acids) in chum salmon Oncorhynchus keta testis were easily determined by reversed-phase high-performance liquid chromatography. For this determination fatty acid methyl esters from triacylglycerols of mature chum salmon testis, containing 14.1% F acid of all the fatty acids, were separated on a reversed-phase column (25 cm × 4.6 mm i. d., 5 μm particles), Supelcosil LC-18. Several μg methyl esters dissolved in acetonitrile were admitted to the column and eluted using acetonitrile. The effluents were monitored at 227 nm UV to detect F acids. Satisfactory separation of F₂, F₄, F₅, F₆ and F₈ acids was acchieved within 20 min. F acid compositions (mol %) were as follows : F₆, 58.6, F₄ 20.1, F₅ 14.4, F₂ 5.2 and F₈ 1.7%. These values were in good agreement with those obtained by gas liquid chromatography.