高度不飽和酸に関する研究 (第2報)
イカ油中の高度不飽和酸部の分別法について
1960 年 9 巻 8 号 p. 415-419
詳細
1960 年 9 巻 8 号 p. 415-419
In order to obtain an available separation method of polyunsaturated fatty acid fractions from cuttle fish oils, following several procedures were comparatively tested;
1) separation by lithium salts from acetone, 2) crystallization from acetone at low temperatures, 3) distillation through spinning band rectifying column at reduced pressure, 4) fractionation by urea adducts of fatty acids from methanol, 5) fractionation by urea adducts of methylesters.
The separation efficiency of these procedures was determined by paper chromatography of reversed phase system consisted of acetonitrile/glacial acetic acid (7/5) as mobile solvent and hydrocarbon as stationary phase and by the yield of polyethenoid fractions.
In the case of the fractionation by urea adducts of methylesters, all of the saturated and lowly unsaturated fractions were removed at room temperature from methanol solutions, and then the highly unsaturated fractions were given at 0°C in the form of adducts. The separation efficiency was the best of five in this case.
