Microanalysis of Triacylglycerol Hydroperoxides by Chemiluminescence-HPLC Assay

Abstract

The optimal conditions of chemiluminescence detection-high performance liquid chromatography (CL-HPLC) method for triacylglycerol hydroperoxide (TGOOH) determination were investigated. Gel permeation chromatography (GPC) column (TSKgel G1000H_XL) was most effective for the TGOOH determination, which can separate hampering α-tocopherol and give a single shape TGOOH peak without influence of fatty acid composition. For olive, safflower, perilla and fish oils, proportional correlationships were confirmed between the peroxide value (PV) and the CL peak area of TGOOH. The present method can determine TGOOH as low as PV 0.04meq/kg in 1mg sample oil and would be useful for studying mechanism of initial rancid reactions in edible oils.