Journal of Oral Science
Online ISSN : 1880-4926
Print ISSN : 1343-4934
ISSN-L : 1343-4934
Original article
Evaluation of in vitro biofilm elimination of Enterococcus faecalis using a continuous ultrasonic irrigation device
Jennifer Galván-PachecoMarlen Vitales-NoyolaAna M. González-AmaroHeriberto Bujanda-WongAntonio Aragón-PiñaVerónica Méndez-GonzálezAmaury Pozos-Guillén
Author information

2020 Volume 62 Issue 4 Pages 415-419


This study sought to evaluate biofilm elimination using the HBW Ultrasonic Ring based on continuous ultrasonic irrigation. Forty-five premolars and molars with complex curvatures were included. An Enterococcus faecalis biofilm was established for 30 days on the extracted teeth. The teeth were then stratified into three experimental groups for instrumentation and irrigation (i.e. HBW Ultrasonic Ring, conventional irrigation, and passive ultrasonic irrigation). Pre- and post-instrumentation samples were collected, and reductions of bacterial load were evaluated by McFarland’s scale, counting of colony-forming units, and scanning electronic microscopy. The HBW Ultrasonic Ring promoted a higher reduction in bacterial load relative to conventional irrigation (P < 0.05) and a similar reduction compared with passive ultrasonic irrigation (P > 0.05). These results suggest the HBW Ultrasonic Ring is a promising alternative modality for simultaneous instrumentation and irrigation during root canal treatment, achieving an appropriate level of bacterial reduction and allowing the passage of the irrigating solution throughout the entire working length.

Content from these authors
© 2020 by Nihon University School of Dentistry
Previous article Next article