Electrophoretic Detection of Salivary α-Amylase Activity

抄録

Fresh samples of human whole saliva containing approximately 20-40μg protein were analyzed using SDS-polyacrylamide slab gel electrophoresis systems. More than 20 protein bands were revealed by Coomassie Brilliant Blue R 250 staining. Some of the protein bands were shown to be glycoprotein-positive with PAS (periodic acid-Schiff) reagent. The protein bands with α-Amylase activity appeared within a molecular weight range of 120, 000-180, 000, which is 2 to 2.8 times higher than the normal molecular weight reported for α-Amylase from parotid saliva, and showed positive staining with PAS reagent. These results show that the α-Amylase in whole saliva appears to exist in a macromolecular form which is not dissociated in the presence of sodium dodecyl sulfate (SDS).