The Journal of Nihon University School of Dentistry
Online ISSN : 1884-2984
Print ISSN : 0029-0432
ISSN-L : 0029-0432
Electrophoretic Detection of Salivary α-Amylase Activity
Zubaidah Hj Abd RAHIMHashim Bin YAACOB
ジャーナル フリー

1992 年 34 巻 4 号 p. 273-277


Fresh samples of human whole saliva containing approximately 20-40μg protein were analyzed using SDS-polyacrylamide slab gel electrophoresis systems. More than 20 protein bands were revealed by Coomassie Brilliant Blue R 250 staining. Some of the protein bands were shown to be glycoprotein-positive with PAS (periodic acid-Schiff) reagent. The protein bands with α-Amylase activity appeared within a molecular weight range of 120, 000-180, 000, which is 2 to 2.8 times higher than the normal molecular weight reported for α-Amylase from parotid saliva, and showed positive staining with PAS reagent. These results show that the α-Amylase in whole saliva appears to exist in a macromolecular form which is not dissociated in the presence of sodium dodecyl sulfate (SDS).

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