The Journal of Nihon University School of Dentistry
Online ISSN : 1884-2984
Print ISSN : 0029-0432
ISSN-L : 0029-0432
Immunohistochemical and biochemical analysis of laminin in neonatal rat first molars
Ricardo Della ColettaSílvio Sanches VeigaSergio Roberto Peres Line
キーワード: laminin isoforms, tooth germ, rat
ジャーナル フリー

1997 年 39 巻 4 号 p. 176-181


Rabbit polyclonal antibody against mouse EHS laminin was used to investigate the distribution and composition of laminin in the rat first molar tooth germ. Immunohistochemical analysis showed that laminin is expressed in the inner and outer epithelia of the enamel organ and in small blood vessels in the dental papilla and strellate reticulum. Immunoblots revealed that tooth germ laminin differs from EHS laminin. Tooth germ laminin contains β chains, while the al chain is substituted by a 300-kDa chain. Two-dimensional electrophoresis analysis of tooth germ extract showed that β chains appeared as four spots with approximate pI values of 6.6, 7.5, 7.8 and 8.5. These results indicate that more than one type of laminin isoform is present in the first molar tooth germ. Additionally, we have shown that despite the early degradation of tooth germ basement membrane, the laminin molecule is still intact at the time of birth.

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