CHROMATOGRAPHY
Online ISSN : 1348-3315
Print ISSN : 1342-8284
ISSN-L : 1342-8284
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LVSEP Analysis of Phosphopeptides in Dynamically PVP-Coated Capillaries and Microchannels
Fumihiko KITAGAWA Ayaka HAYASHIIsoshi NUKATSUKA
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2022 Volume 43 Issue 1 Pages 37-41

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Abstract

To achieve highly sensitive analyses of phosphopeptides with simple experimental procedures in capillary electrophoresis (CE) and microchip electrophoresis (MCE), large-volume sample stacking with an electroosmotic flow pump (LVSEP) was performed in dynamically poly(vinyl pyrrolidone) (PVP)-coated capillaries to suppress the electroosmotic flow. In the analysis of monophosphopeptide (MPP) from bovine β-casein, good enrichments were attained with the sensitive enhancement factor (SEF) of 260 and 400 in CE and MCE, respectively, by using 0.2% PVP in 20 mM HEPES buffer (pH 9.0) in LVSEP.

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© 2022 The Society for Chromatographic Sciences
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